Review



retrograde aav expressing mcherry  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    Addgene inc retrograde aav expressing mcherry
    (A) Experimental timeline. (B) Bilateral virus infusion schematics. Cre-dependent, EYFP-tagged channelrhodopsin (DIO-ChR2-EYFP) was infused into the nucleus accumbens shell (NAcS), and <t>retrograde</t> <t>AAV</t> carrying <t>mCherry</t> was infused into the ventral tegmental area (VTA). Ex vivo electrophysiology recording was performed in the ventral pallidum (VP). (C) Representative live brain slice images are shown demonstrating ChR2 and retrograde signals converging in the ventromedial region of VP (yellow circle). (D) Representative traces of asynchronous inhibitory postsynaptic currents (aIPSC). Yellow box denotes the 50 - 550 ms analysis window after optogenetic stimulation (cyan). (E) Average aIPSC frequency was suppressed by cocaine abstinence in Adora2a-Cre controls (saline: 19 cells/10 mice, cocaine: 10 cells/5 mice). Cocaine-abstinence-induced aIPSC suppression was not observed in D2-PenkKOs (saline: 14 cells/5 mice, cocaine: 10 cells/5 mice). Bath application of CTAP (1 µM) did not affect aIPSC frequency. (F) Average aIPSC amplitude was not affected by cocaine abstinence, deletion of striatal enkephalin, or CTAP. Data are shown as mean ± SEM, with individual data points labeled as male (circles) and females (triangles). ** p < 0.01.
    Retrograde Aav Expressing Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 97/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/retrograde+aav+expressing+mcherry/bio_rxiv__64898__2026__03__11__711212-45-5-10?v=Addgene+inc
    Average 97 stars, based on 168 article reviews
    retrograde aav expressing mcherry - by Bioz Stars, 2026-08
    97/100 stars

    Images

    1) Product Images from "Enkephalin Gates D2-MSN Disinhibition of the Ventral Pallidum During Cocaine Abstinence"

    Article Title: Enkephalin Gates D2-MSN Disinhibition of the Ventral Pallidum During Cocaine Abstinence

    Journal: bioRxiv

    doi: 10.64898/2026.03.11.711212

    (A) Experimental timeline. (B) Bilateral virus infusion schematics. Cre-dependent, EYFP-tagged channelrhodopsin (DIO-ChR2-EYFP) was infused into the nucleus accumbens shell (NAcS), and retrograde AAV carrying mCherry was infused into the ventral tegmental area (VTA). Ex vivo electrophysiology recording was performed in the ventral pallidum (VP). (C) Representative live brain slice images are shown demonstrating ChR2 and retrograde signals converging in the ventromedial region of VP (yellow circle). (D) Representative traces of asynchronous inhibitory postsynaptic currents (aIPSC). Yellow box denotes the 50 - 550 ms analysis window after optogenetic stimulation (cyan). (E) Average aIPSC frequency was suppressed by cocaine abstinence in Adora2a-Cre controls (saline: 19 cells/10 mice, cocaine: 10 cells/5 mice). Cocaine-abstinence-induced aIPSC suppression was not observed in D2-PenkKOs (saline: 14 cells/5 mice, cocaine: 10 cells/5 mice). Bath application of CTAP (1 µM) did not affect aIPSC frequency. (F) Average aIPSC amplitude was not affected by cocaine abstinence, deletion of striatal enkephalin, or CTAP. Data are shown as mean ± SEM, with individual data points labeled as male (circles) and females (triangles). ** p < 0.01.
    Figure Legend Snippet: (A) Experimental timeline. (B) Bilateral virus infusion schematics. Cre-dependent, EYFP-tagged channelrhodopsin (DIO-ChR2-EYFP) was infused into the nucleus accumbens shell (NAcS), and retrograde AAV carrying mCherry was infused into the ventral tegmental area (VTA). Ex vivo electrophysiology recording was performed in the ventral pallidum (VP). (C) Representative live brain slice images are shown demonstrating ChR2 and retrograde signals converging in the ventromedial region of VP (yellow circle). (D) Representative traces of asynchronous inhibitory postsynaptic currents (aIPSC). Yellow box denotes the 50 - 550 ms analysis window after optogenetic stimulation (cyan). (E) Average aIPSC frequency was suppressed by cocaine abstinence in Adora2a-Cre controls (saline: 19 cells/10 mice, cocaine: 10 cells/5 mice). Cocaine-abstinence-induced aIPSC suppression was not observed in D2-PenkKOs (saline: 14 cells/5 mice, cocaine: 10 cells/5 mice). Bath application of CTAP (1 µM) did not affect aIPSC frequency. (F) Average aIPSC amplitude was not affected by cocaine abstinence, deletion of striatal enkephalin, or CTAP. Data are shown as mean ± SEM, with individual data points labeled as male (circles) and females (triangles). ** p < 0.01.

    Techniques Used: Virus, Ex Vivo, Slice Preparation, Saline, Labeling



    Similar Products

    retrograde aav expressing mcherry  (Addgene inc)
    97
    Addgene inc retrograde aav expressing mcherry
    (A) Experimental timeline. (B) Bilateral virus infusion schematics. Cre-dependent, EYFP-tagged channelrhodopsin (DIO-ChR2-EYFP) was infused into the nucleus accumbens shell (NAcS), and <t>retrograde</t> <t>AAV</t> carrying <t>mCherry</t> was infused into the ventral tegmental area (VTA). Ex vivo electrophysiology recording was performed in the ventral pallidum (VP). (C) Representative live brain slice images are shown demonstrating ChR2 and retrograde signals converging in the ventromedial region of VP (yellow circle). (D) Representative traces of asynchronous inhibitory postsynaptic currents (aIPSC). Yellow box denotes the 50 - 550 ms analysis window after optogenetic stimulation (cyan). (E) Average aIPSC frequency was suppressed by cocaine abstinence in Adora2a-Cre controls (saline: 19 cells/10 mice, cocaine: 10 cells/5 mice). Cocaine-abstinence-induced aIPSC suppression was not observed in D2-PenkKOs (saline: 14 cells/5 mice, cocaine: 10 cells/5 mice). Bath application of CTAP (1 µM) did not affect aIPSC frequency. (F) Average aIPSC amplitude was not affected by cocaine abstinence, deletion of striatal enkephalin, or CTAP. Data are shown as mean ± SEM, with individual data points labeled as male (circles) and females (triangles). ** p < 0.01.
    Retrograde Aav Expressing Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/retrograde+aav+expressing+mcherry/bio_rxiv__64898__2026__03__11__711212-45-5-10?v=Addgene+inc
    Average 97 stars, based on 1 article reviews
    retrograde aav expressing mcherry - by Bioz Stars, 2026-08
    97/100 stars
    		  Buy from Supplier
    aav retrograde virus expressing paav hsyn hm4d gi mcherry  (Addgene inc)
    96
    Addgene inc aav retrograde virus expressing paav hsyn hm4d gi mcherry
    (A) Experimental timeline. (B) Bilateral virus infusion schematics. Cre-dependent, EYFP-tagged channelrhodopsin (DIO-ChR2-EYFP) was infused into the nucleus accumbens shell (NAcS), and <t>retrograde</t> <t>AAV</t> carrying <t>mCherry</t> was infused into the ventral tegmental area (VTA). Ex vivo electrophysiology recording was performed in the ventral pallidum (VP). (C) Representative live brain slice images are shown demonstrating ChR2 and retrograde signals converging in the ventromedial region of VP (yellow circle). (D) Representative traces of asynchronous inhibitory postsynaptic currents (aIPSC). Yellow box denotes the 50 - 550 ms analysis window after optogenetic stimulation (cyan). (E) Average aIPSC frequency was suppressed by cocaine abstinence in Adora2a-Cre controls (saline: 19 cells/10 mice, cocaine: 10 cells/5 mice). Cocaine-abstinence-induced aIPSC suppression was not observed in D2-PenkKOs (saline: 14 cells/5 mice, cocaine: 10 cells/5 mice). Bath application of CTAP (1 µM) did not affect aIPSC frequency. (F) Average aIPSC amplitude was not affected by cocaine abstinence, deletion of striatal enkephalin, or CTAP. Data are shown as mean ± SEM, with individual data points labeled as male (circles) and females (triangles). ** p < 0.01.
    Aav Retrograde Virus Expressing Paav Hsyn Hm4d Gi Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/retrograde+aav+expressing+mcherry/10__1523_slash_jneurosci__1388___23__2023-52-4-9?v=Addgene+inc
    Average 96 stars, based on 1 article reviews
    aav retrograde virus expressing paav hsyn hm4d gi mcherry - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier
    aav retrograde virus expressing hsyn hm4d i  (Addgene inc)
    96
    Addgene inc aav retrograde virus expressing hsyn hm4d i
    (A) Experimental timeline. (B) Bilateral virus infusion schematics. Cre-dependent, EYFP-tagged channelrhodopsin (DIO-ChR2-EYFP) was infused into the nucleus accumbens shell (NAcS), and <t>retrograde</t> <t>AAV</t> carrying <t>mCherry</t> was infused into the ventral tegmental area (VTA). Ex vivo electrophysiology recording was performed in the ventral pallidum (VP). (C) Representative live brain slice images are shown demonstrating ChR2 and retrograde signals converging in the ventromedial region of VP (yellow circle). (D) Representative traces of asynchronous inhibitory postsynaptic currents (aIPSC). Yellow box denotes the 50 - 550 ms analysis window after optogenetic stimulation (cyan). (E) Average aIPSC frequency was suppressed by cocaine abstinence in Adora2a-Cre controls (saline: 19 cells/10 mice, cocaine: 10 cells/5 mice). Cocaine-abstinence-induced aIPSC suppression was not observed in D2-PenkKOs (saline: 14 cells/5 mice, cocaine: 10 cells/5 mice). Bath application of CTAP (1 µM) did not affect aIPSC frequency. (F) Average aIPSC amplitude was not affected by cocaine abstinence, deletion of striatal enkephalin, or CTAP. Data are shown as mean ± SEM, with individual data points labeled as male (circles) and females (triangles). ** p < 0.01.
    Aav Retrograde Virus Expressing Hsyn Hm4d I, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/retrograde+aav+expressing+mcherry/bio_rxiv__2023__04__28__538437-28-4-10?v=Addgene+inc
    Average 96 stars, based on 1 article reviews
    aav retrograde virus expressing hsyn hm4d i - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Experimental timeline. (B) Bilateral virus infusion schematics. Cre-dependent, EYFP-tagged channelrhodopsin (DIO-ChR2-EYFP) was infused into the nucleus accumbens shell (NAcS), and retrograde AAV carrying mCherry was infused into the ventral tegmental area (VTA). Ex vivo electrophysiology recording was performed in the ventral pallidum (VP). (C) Representative live brain slice images are shown demonstrating ChR2 and retrograde signals converging in the ventromedial region of VP (yellow circle). (D) Representative traces of asynchronous inhibitory postsynaptic currents (aIPSC). Yellow box denotes the 50 - 550 ms analysis window after optogenetic stimulation (cyan). (E) Average aIPSC frequency was suppressed by cocaine abstinence in Adora2a-Cre controls (saline: 19 cells/10 mice, cocaine: 10 cells/5 mice). Cocaine-abstinence-induced aIPSC suppression was not observed in D2-PenkKOs (saline: 14 cells/5 mice, cocaine: 10 cells/5 mice). Bath application of CTAP (1 µM) did not affect aIPSC frequency. (F) Average aIPSC amplitude was not affected by cocaine abstinence, deletion of striatal enkephalin, or CTAP. Data are shown as mean ± SEM, with individual data points labeled as male (circles) and females (triangles). ** p < 0.01.

    Journal: bioRxiv

    Article Title: Enkephalin Gates D2-MSN Disinhibition of the Ventral Pallidum During Cocaine Abstinence

    doi: 10.64898/2026.03.11.711212

    Figure Lengend Snippet: (A) Experimental timeline. (B) Bilateral virus infusion schematics. Cre-dependent, EYFP-tagged channelrhodopsin (DIO-ChR2-EYFP) was infused into the nucleus accumbens shell (NAcS), and retrograde AAV carrying mCherry was infused into the ventral tegmental area (VTA). Ex vivo electrophysiology recording was performed in the ventral pallidum (VP). (C) Representative live brain slice images are shown demonstrating ChR2 and retrograde signals converging in the ventromedial region of VP (yellow circle). (D) Representative traces of asynchronous inhibitory postsynaptic currents (aIPSC). Yellow box denotes the 50 - 550 ms analysis window after optogenetic stimulation (cyan). (E) Average aIPSC frequency was suppressed by cocaine abstinence in Adora2a-Cre controls (saline: 19 cells/10 mice, cocaine: 10 cells/5 mice). Cocaine-abstinence-induced aIPSC suppression was not observed in D2-PenkKOs (saline: 14 cells/5 mice, cocaine: 10 cells/5 mice). Bath application of CTAP (1 µM) did not affect aIPSC frequency. (F) Average aIPSC amplitude was not affected by cocaine abstinence, deletion of striatal enkephalin, or CTAP. Data are shown as mean ± SEM, with individual data points labeled as male (circles) and females (triangles). ** p < 0.01.

    Article Snippet: In the same surgery, a retrograde AAV expressing mCherry (rgAAV.hSyn.mCherry, Addgene Catalog#: 114472-AAVrg, RRID: Addgene_114472, titer: 9.5 x 10 12 , 200 nL/side) was bilaterally infused (from bregma in mm: AP -3.15, ML ± 0.4, DV -4.75) into the ventral tegmental area (VTA) to identify vmVP neurons projecting to the VTA.

    Techniques: Virus, Ex Vivo, Slice Preparation, Saline, Labeling